Chlorophyll a extraction from Chlorella Vulgaris - Bertin Instruments

Environnement

Chlorophyll a extraction from Chlorella Vulgaris

Sources: Institute of Environmental Technology and Industry, Pusan National University, South Korea

Context

This laboratory is working on the development of an efficient maintenance strategy for stream ecosystems. This study focuses on the extraction of Chlorophyll a from Chlorella vulgarisand compares two different homogenization methods : the Precellys® bead-beater and a hand-held, Con-Torque Tissue Homogenizer.

Results

The  total  yield  of  Chlorophyll  a  extracted  is  higher  with Precellys compared to the manual grinding method (Fig. 1).

Precellys ®  24-Dual  :  11,500  µg/L  (average  of  triplicate values)

Hand  grind  method  :  7,000  µg/L  (average  of  triplicate values)

image19

 

 

Figure  1  :  Comparison  of  chlorophyll  a concentration obtained with two different homogenization methods : the Precellys ®   24-Dual and a manual tissue homogenizer.

 

 

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