DNA extraction from plant leaves with Minilys - Bertin Instruments

    Environnement

    DNA extraction from plant leaves with Minilys

    Context

    Minilys is a small and compact homogenizer for fast and hand-free disruption of a wide range of biological samples prior extraction of DNA, RNA, proteins or drugs. Based on 3D motion bead-beating, Minilys homogenizes, grinds, dissociates samples into 0.5mL, 2mL or 7mL tubes. The mechanical sample prep is compatible with any analysis DNA-RNA electrophoresis, Real time PCR, Western-blot, GC/MS, LC/MS. In this application, the homogenization of plant leaves to extract DNA is illustrated.

    Results

    Concentration of DNA obtained from plant leaves after homogenization with Minilys and absorption ratios were determined and shown in the figure 1. Equivalent total genomic DNA is obtained with Minilys homogenizer. High quality DNA is obtained from all extracted samples.

    Figure 1: mean concentration of total genomic DNA from 3 extracted samples of leaves with Minilys (run 1 and 2) in comparison with Precellys. The corresponding mean absorption ratios (260/280; 260/230) are also plotted.

    Figure 1: mean concentration of total genomic DNA from 3 extracted samples of leaves with Minilys (run 1 and 2) in comparison with Precellys. The corresponding mean absorption ratios (260/280; 260/230) are also plotted.

     

    Figure 2: illustration of NanoDrop analysis.

    Figure 2: illustration of NanoDrop analysis.

     

    Figure 3: sample preparation.

    Figure 3: sample preparation.

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