DNA Extraction from Rice Grain - Bertin Instruments

Agronomie

DNA Extraction from Rice Grain

Sources: Kogene Biotech Co – South Korea

Context

Rice is a key element of the Korean food. Due to Korean regulations, the rice species need to be defined and evaluated. Therefore, DNA qualification and classification are essential. In this study, a rice grain specie is identified using Precellys®24 vs. mortar.

Results

Grinding efficiency: Rice grains of 3 species were grinded into fine powder following the protocol described. DNA analysis: Rice grains (Orysa sartiva L.) were grinded by Precellys®24 and a mortar. DNA was extracted and loaded directly onto an agarose gel after PCR amplification. Both Precellys®24 and mortar are efficient to extract a good quality of DNA. The DNA quantity from rice grains grinded by Precellys®24 (P1, P2,P3 and P4) are similar to the result with a mortar (M1, M2, M3 and M4).
dna extraction from rice grain
Precellys®24 reduces into powder any species of rice grain in 1 minute.

 

Precellys®24 gives a faster, easier and more consistent sample preparation with results as efficient as mortar.

Precellys®24 gives a faster, easier and more consistent sample preparation with results as efficient as mortar.

 

Rice (Orysa sartiva L.).

Rice (Orysa sartiva L.).

 

 

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