DNA Extraction from Rice Leaf - Bertin Instruments

    Agronomie

    DNA Extraction from Rice Leaf

    Sources: National Institute of Agrobiological Sciences - Japan

    Context

    The research plan is to isolate the genes of functional proteins and clarify their functions by introducing them into plants. The lab is studying the gene transfection into a plant seed by electroporation. Our team checks how much volume of DNA is introduced into a seed by a standard molecular biology process.

    Results

    DNA was extracted with Nucleon™ PhytoPure™ Genomic DNA Extraction Kits from GE Helthcare. The migration gives the following results : (Lane No.1 and No.2 are replications). Since the concentration of the DNA is known, it is used to semi-quantify the amount of extracted DNA by visual observation.

    DNA extraction rice leaf

    Fresh leaves, before LN

     

    Frozen leaves after grinding

    Frozen leaves after grinding

     

    1. DNA from rice leaf 2. DNA from rice leaf 3. ?DNA 0.05µg 4. ?DNA 0.1µg 5. ?DNA 0.5µg 6. Marker 7. Marker

    1. DNA from rice leaf 2. DNA from rice leaf 3. ?DNA 0.05µg 4. ?DNA 0.1µg 5. ?DNA 0.5µg 6. Marker 7. Marker

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