Environmental Biomarkers Analysis - Bertin Instruments

Environnement

Environmental Biomarkers Analysis

Sources: Laboratory of Industrial and Environmental Toxicology ULCO - Dunkerque / ILIS - Lille II

Context

Mosses are environmental biomarkers used for the long term accumulation and bioaccumulation assessment of atmospheric particles. Organic and inorganic pollutants could be responsible for oxidative stress and biological modifications. The biomarkers are here the MDA concentration by HPLC (MalonDiAldehyde resulting from membranes lipoperoxidation), the 8 hydroxy-2- deoxyguanosine concentration by ELISA (DNA adducts) and the DNA fragmentation (DNA ladder, results shown)

Results

In situ exposures proceed on 2 sites: a site mainly contaminated by industrial emissions (Dunkerque, North of France) and rural site (Montagney, East of France). During exposures, sampling is carried out every 8 weeks delimiting 3 period exposure : T1, T2, T3 and T0 corresponding to nonexposed mosses. The “smear” observed indicated the DNA fragmentation. The DNA ladder test seems to reveal DNA fragmentation for samples exposed in industrial site for 16 and 24 months.

 

img-03961-006-DU002

 

img-03961-006-DU002-2

DES QUESTIONS ? CONTACTER UN EXPERT

Remplissez ce formulaire pour être rappelé par notre équipe commerciale.

Mon compte
Mot de passe oublié ?

Ajouter une question !

Ajouter une question