Environmental Biomarkers Analysis - Bertin Instruments

    Environnement

    Environmental Biomarkers Analysis

    Sources: Laboratory of Industrial and Environmental Toxicology ULCO - Dunkerque / ILIS - Lille II

    Context

    Mosses are environmental biomarkers used for the long term accumulation and bioaccumulation assessment of atmospheric particles. Organic and inorganic pollutants could be responsible for oxidative stress and biological modifications. The biomarkers are here the MDA concentration by HPLC (MalonDiAldehyde resulting from membranes lipoperoxidation), the 8 hydroxy-2- deoxyguanosine concentration by ELISA (DNA adducts) and the DNA fragmentation (DNA ladder, results shown)

    Results

    In situ exposures proceed on 2 sites: a site mainly contaminated by industrial emissions (Dunkerque, North of France) and rural site (Montagney, East of France). During exposures, sampling is carried out every 8 weeks delimiting 3 period exposure : T1, T2, T3 and T0 corresponding to nonexposed mosses. The “smear” observed indicated the DNA fragmentation. The DNA ladder test seems to reveal DNA fragmentation for samples exposed in industrial site for 16 and 24 months.

     

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