HOMOGENIZATION AND EXTRACTION OF FLUORESCENT CHEMICAL COMPOUNDS FROM MOUSE BONE TISSUE - Bertin Instruments

    Pharmacologie

    HOMOGENIZATION AND EXTRACTION OF FLUORESCENT CHEMICAL COMPOUNDS FROM MOUSE BONE TISSUE

    Sources: Atlanthera Laboratories, Saint-Herblain, France

    Context

    Atlanthera is a drug discovery company specialized in bone-targeted drug delivery through covalent binding of drugs to hydroxybisphosphonates (HBP), a class of molecule known for high affinity to bone. Extraction of HBP-conjugated chemical from bone is challenging and require efficient tissue homogenization. Precellys® Evolution system was tested for bone homogenization and compared with a cryogenic grinder.

    Results

    The best homogenization efficiency was obtained at 8500 rpm on dry rather than homogenization in buffer.
    Compound extraction was equivalent to our standard protocol (cryogenic grinder)

    SPPP-810-DU124_Mouse bone

    Figure 1: Example for HPLC analysis of compound extracted from bone tissue after homogenization with Precellys® Evolution

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