Improving RNA Extraction from Mouse Tissues using the Precellys 24-Dual - Bertin Instruments

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Improving RNA Extraction from Mouse Tissues using the Precellys 24-Dual

Sources: Pharmaceutical Company, San Francisco, California

Context

The time spent on sample processing is often time consuming and arduous when using traditional methods, particularly for large scale animal studies. In our research, mice organs are dissected after small molecule treatment for RNA extraction, followed by cDNA synthesis and gene expression analysis using real time PCR. Our current pulverization method results in poor gene expression results due to RNA degradation during sample extraction. We aim to improve the quality of RNA yield using the Precellys24-Dual, a high throughput sample homogenizer.

Results

RNA integrity and yield was evaluated using an Agilent Bioanalyzer, and an average RIN (RNA integrity number) value of 9 (highest score is 10) was observed using the Precellys, with RNA concentration falling between 400-1800 ng/ul. The gel displays clean 18S and 28S rRNA bands with little or no degradation (Figure 1A). RIN values of “N/A” were observed after pulverization, which is indicative of highly degraded RNA as observed on the gel (Figure 1B).

 

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