In an animal model of invasive infections caused by Streptococcus pyogenes, bacteria will colonize various tissues and organs. Bacterial loads present in these tissues is determined by homogenization, serial dilution and plating onto nutrient agar. This study investigates the use of the Precellys®24for tissue homogenization.
Following tissue homogenization, the number of viable bacterial cells (CFU/ml) present was determined by serial dilution in sterile 0.7% saline and plating onto nutrient agar. Results (see Figure1) indicate that for all treatment groups the type of bead and the processing time had no significant effect on the viability of S. pyogenes (p =0.1).