Prion Protein Extraction in Animal Tissues - Bertin Instruments

    Biomédical et santé

    Prion Protein Extraction in Animal Tissues

    Sources: URI282 - Animal Infectiology and Public Health National Institute of Agronomic Research of Nouzilly

    Context

    In TSE, the preparation of animal tissues is an important step for the detection of the pathological prion protein. Brain and spinal cord tissues, lymphoid tissues (tonsils, lymph nodes, spleen…) are frozen at -80°C. The samples are suspended in a buffer, and protein extraction is followed by ELISA analysis and Western Blot Analysis.

    Results

    After prion protein extraction, the pathologic protein is detected and quantified by using specific antibodies through an ELISA test, and the pattern of the glycozylated protein is analyzed by Western Blot.

     

    ELISA analysis.

    ELISA analysis.

     

    Lanes 4-6 : two positive references. Lanes 1-3 : BSE or Scrapie infected brain tissues. Lanes 5,7,8 : BSE infected lymphoid tissues.

    Lanes 4-6 : two positive references. Lanes 1-3 : BSE or Scrapie infected brain tissues. Lanes 5,7,8 : BSE infected lymphoid tissues.

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