This laboratory develops biomolecular solutions for plant biomass production. The proteins extraction from methylotrophic yeast is a part of the laboratory’s daily work.
The following treatment 4000 rpm 20 sec x 2 already gave good extraction of protein. There were no sign of the excess disintegration of the proteins even after 5000 rpm 20 sec x 6 treatment. Even the 5000 rpm 20 sec x 6 treatment gave no excess heat and the tube was less than 30°C after each treatment.