Protein extraction from yeast Pichia pastoris with Minilys - Bertin Instruments

    Microbiologie

    Protein extraction from yeast Pichia pastoris with Minilys

    Sources: Tokyo University of Agricultural and Technology, Institute ofAgriculture, Division of Bio-regulation and Bio-interaction, Japan

    Context

    This laboratory develops biomolecular solutions for plant biomass production. The proteins extraction from methylotrophic yeast is a part of the laboratory’s daily work.

    Results

    The following treatment 4000 rpm 20 sec x 2 already gave good extraction of protein. There were no sign of the excess disintegration of the proteins even after 5000 rpm 20 sec x 6 treatment. Even the 5000 rpm 20 sec x 6 treatment gave no excess heat and the tube was less than 30°C after each treatment.

     

    SDS-PAGE of the protein extracted from Pichia pastoris.

    SDS-PAGE of the protein extracted from Pichia pastoris.

     

     

    DES QUESTIONS ? CONTACTER UN EXPERT

    Remplissez ce formulaire pour être rappelé par notre équipe commerciale.

    Mon compte
    Mot de passe oublié ?

    Ajouter une question !

    Ajouter une question