RNA extraction from mouse embryonic tooth germs in 0.5mL vial - Bertin Instruments

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    RNA extraction from mouse embryonic tooth germs in 0.5mL vial

    Sources: Functional Genomics Institute of Lyon (France)

    Context

    Our team aims at better understanding how a morphology arises during development in connection with gene expression, and how it changes during evolution. For that purpose, we are using the rodent molar as an organ model. In order to study gene expression during tooth development, developing tooth germs (soft tissue) were dissected from mouse embryos (15-16 days post coïtum) and stored in RNA later.

    Results

    RNA extracted was of very good quality (RIN = 10). The yield was closed to theoretical yield for embryonic tissues (theoretical: 1.5mg; obtained: 1 to 1.4 mg). Note: Increasing lysis time (5500 rpm 2x 20s (10s)) did not lead to any yield improvement.

    typical profile obtained on Agilent Bioalnalyser 2100 following lysis with Precellys®24 and purification with Qiagen RNeasy micro kit.

    typical profile obtained on Agilent Bioalnalyser 2100 following lysis with Precellys®24 and purification with Qiagen RNeasy micro kit.

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