The “scratch” assay is the most widely used as it is a simple, well established, and inexpensive method to measure cell migration in vitro.
The basic steps involve creating a « scratch » in a cell monolayer, capturing the images at the beginning and at regular intervals during cell migration to close the scratch, and comparing the images to quantify the migration rate of the cells. It is often applied to study cell migration, drug effects on cells, angiogenesis, cell-cell interactions, metastasis, woundhealing…
To ideally follow the scratchtest, alive cell imaging is often use, but only few labs own a live cell imaging system. The InCellis®, with the cell confluency application, offers the best alternative to follow the results of a scratch test, as the cell confluency % will increase within the cell growth and can be correlate to the cell migration rate.